Hotspot

atpI-atpH

LSC · 31460-32300 · 841 bp

Back to Stipagrostis

Pi 0.00556

MarkerSeek score 59.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 841
variable_sites: 7
pi: 0.00556
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	AGCCAATCCAGCAGCAATAA	45.0	45.0	57.9	57.9	942-946 bp	1.0	79.3
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	45.0	55.0	57.9	58.2	986-990 bp	1.0	78.9
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.9	57.8	919-923 bp	1.0	78.9
atpI-atpH_p4	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	45.0	50.0	57.9	57.8	941-945 bp	1.0	78.8
atpI-atpH_p5	TTTTGCAACATTAGCCGCAG	ATAACGGAAGCAGCAGCAAT	45.0	45.0	57.9	58.2	926-930 bp	1.0	78.6