Hotspot

rpoC2-rps2

LSC · 29432-29757 · 326 bp

Back to Stipagrostis

Pi 0.0327

MarkerSeek score 65.60

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 12
pi: 0.0327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	AACCCCCATTTACCCCCATT	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.9	58.2	453-485 bp	1.0	77.3
rpoC2-rps2_p2	ACCCCCATTTACCCCCATTT	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.9	58.2	452-484 bp	1.0	77.3
rpoC2-rps2_p3	AACCCCCATTTACCCCCATT	GCTTTGCCGAGATGTAAGGT	50.0	50.0	58.9	58.3	448-480 bp	1.0	76.8
rpoC2-rps2_p4	ACCCCCATTTACCCCCATTT	GCTTTGCCGAGATGTAAGGT	50.0	50.0	58.9	58.3	447-479 bp	1.0	76.8
rpoC2-rps2_p5	TCAGAACCCCCATTTACCCC	TTTACGCTTTGCCGAGATGT	55.0	45.0	59.0	58.2	457-489 bp	1.0	76.5