Hotspot

trnY-GUA-trnD-GUC

LSC · 14735-15108 · 374 bp

Back to Stipagrostis

Pi 0.0107

MarkerSeek score 60.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 6
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AAATCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	498-506 bp	1.0	76.8
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	465-473 bp	1.0	74.4
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	464-472 bp	1.0	74.2
trnY-GUA-trnD-GUC_p4	AAATCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	497-505 bp	1.0	72.6
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	466-474 bp	1.0	71.0