Hotspot

rbcL

LSC · 54983-56413 · 1431 bp

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Pi 0.0064
MarkerSeek score 37.70
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1431
variable_sites
51
pi
0.0064
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 AGCCCGTTGGAAAGTTCAAT CTCACGATTCCGCCTGTTAA 45.0 50.0 58.0 58.0 2671-2694 bp 0.98 84.1
rbcL_p2 AATGAGCCCGTTGGAAAGTT CTCACGATTCCGCCTGTTAA 45.0 50.0 58.0 58.0 2675-2698 bp 0.98 84.0
rbcL_p3 TTGTGGAGGATCTGTGCCTA CTCACGATTCCGCCTGTTAA 50.0 50.0 58.0 58.0 2623-2646 bp 0.98 83.8
rbcL_p4 AATGAGCCCGTTGGAAAGTT CTTACACGCAGTTCGAGTCA 45.0 50.0 58.0 58.0 2703-2726 bp 0.96 83.1
rbcL_p5 AGCCCGTTGGAAAGTTCAAT CTTACACGCAGTTCGAGTCA 45.0 50.0 58.0 58.0 2699-2722 bp 0.96 83.1