Hotspot

ndhF

SSC · 102971-105190 · 2220 bp

Pi 0.00444

MarkerSeek score 43.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 98
pi: 0.00444
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGGATAAGGAGGGGGATA	GAATTGGTTTGGTGAATCGGA	55.0	42.9	58.1	56.8	2356-2365 bp	0.84	57.4
ndhF_p2	AGGGGATAAGGAGGGGGATA	AGAATTGGTTTGGTGAATCGG	55.0	42.9	58.1	56.8	2357-2366 bp	0.84	57.3
ndhF_p3	AGGGGATAAGGAGGGGGATA	TTGGTTTGGTGAATCGGAAA	55.0	40.0	58.1	55.8	2353-2362 bp	0.84	57.1
ndhF_p4	AGGGGATAAGGAGGGGGATA	AGAATTGGTTTGGTGAATCGGA	55.0	40.9	58.1	58.2	2357-2366 bp	0.84	57.1
ndhF_p5	GGGATAAGGAGGGGGATAGG	GAATTGGTTTGGTGAATCGGA	60.0	42.9	57.7	56.8	2354-2363 bp	0.84	56.1