Hotspot

ndhA

SSC · 112604-114725 · 2122 bp

Pi 0.0029

MarkerSeek score 52.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2122
variable_sites: 70
pi: 0.0029
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2258-2279 bp	0.98	72.0
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2256-2277 bp	0.98	71.5
ndhA_p3	TGAACCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	57.8	57.9	2261-2282 bp	0.98	71.0
ndhA_p4	TGAACCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	57.8	57.8	2259-2280 bp	0.98	70.5
ndhA_p5	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2257-2278 bp	0.98	66.4