Hotspot

ndhF-rpl32

SSC · 105191-106107 · 917 bp

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Pi 0.00778
MarkerSeek score 60.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
917
variable_sites
72
pi
0.00778
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 968-1016 bp 1.0 50.0
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 969-1017 bp 1.0 50.0
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 995-1043 bp 1.0 49.9
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 970-1018 bp 1.0 49.9
ndhF-rpl32_p5 GGCCAAACCCCATTGACATA TCCAAATATTTTTACGAATACGCT 50.0 29.2 58.1 55.3 1021-1069 bp 1.0 49.8