Hotspot

rpl22-rps19

IRb · 81171-81254 · 84 bp

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Pi 0.00751
MarkerSeek score 53.30
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
84
variable_sites
6
pi
0.00751
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCCTTGCGAGTTTCCTTAGC CAAATTGGGGGAATTCGTGC 50.0 50.0 57.9 58.0 231-241 bp 1.0 87.2
rpl22-rps19_p2 TCCTTGCGAGTTTCCTTAGC GCAAATTGGGGGAATTCGTG 50.0 50.0 57.9 58.0 232-242 bp 1.0 87.2
rpl22-rps19_p3 TGAGTAGACGATCGGTTGGA CAAATTGGGGGAATTCGTGC 50.0 50.0 57.9 58.0 255-265 bp 1.0 86.9
rpl22-rps19_p4 TGAGTAGACGATCGGTTGGA GCAAATTGGGGGAATTCGTG 50.0 50.0 57.9 58.0 256-266 bp 1.0 86.9
rpl22-rps19_p5 TCCTTGCGAGTTTCCTTAGC ATGGTAGGTCGCAAATTGGG 50.0 50.0 57.9 58.2 242-252 bp 1.0 85.5