Hotspot

rps3-rpl22

LSC · 80665-80720 · 56 bp

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Pi 0.00634
MarkerSeek score 49.10
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
56
variable_sites
6
pi
0.00634
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 ACCAAGTCTGAAACCGAGTG AAAATTCAGACCTCGGGCTC 50.0 50.0 57.8 57.9 192-197 bp 0.98 80.1
rps3-rpl22_p2 CCAAGTCTGAAACCGAGTGG AAAATTCAGACCTCGGGCTC 55.0 50.0 58.5 57.9 191-196 bp 1.0 79.4
rps3-rpl22_p3 CAAGTCTGAAACCGAGTGGA AAAATTCAGACCTCGGGCTC 50.0 50.0 57.5 57.9 190-195 bp 1.0 79.1
rps3-rpl22_p4 ACCAAGTCTGAAACCGAGTG AAAAATTCAGACCTCGGGCT 50.0 45.0 57.8 57.4 193-198 bp 0.98 76.3
rps3-rpl22_p5 CGCGAACCAAAAGGAATGAT AAAATTCAGACCTCGGGCTC 45.0 50.0 57.1 57.9 225 bp 0.96 71.5