Hotspot

infA-rps8

LSC · 77208-77318 · 111 bp

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Pi 0.0197
MarkerSeek score 62.20
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
111
variable_sites
12
pi
0.0197
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 TGACTTTTGCTTCTCTCGGG GGGTGGAATGGGAATTGCTA 50.0 50.0 57.8 57.5 255-272 bp 1.0 83.7
infA-rps8_p2 GACTTTTGCTTCTCTCGGGT GGGTGGAATGGGAATTGCTA 50.0 50.0 57.8 57.5 254-271 bp 1.0 83.7
infA-rps8_p3 AACATTCCGTTGGGTAGAGC GGGTGGAATGGGAATTGCTA 50.0 50.0 57.9 57.5 298-315 bp 1.0 83.6
infA-rps8_p4 TGGGTAGAGCTTCCGTAACT GGGTGGAATGGGAATTGCTA 50.0 50.0 57.8 57.5 288-305 bp 1.0 82.8
infA-rps8_p5 AACATTCCGTTGGGTAGAGC TTGGGTGGAATGGGAATTGC 50.0 50.0 57.9 58.7 300-317 bp 1.0 81.4