Hotspot

trnW-CCA-trnP-UGG

LSC · 64629-64767 · 139 bp

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Pi 0.00576
MarkerSeek score 50.00
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
139
variable_sites
10
pi
0.00576
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT CTTGGTAGCGCGTTTGTTTT 50.0 45.0 57.9 57.9 222-226 bp 1.0 83.9
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT TTGGTAGCGCGTTTGTTTTG 50.0 45.0 57.9 58.2 221-225 bp 1.0 83.5
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT CGCGTTTGTTTTGGGTACAA 50.0 45.0 57.9 57.8 214-218 bp 1.0 83.5
trnW-CCA-trnP-UGG_p4 ACCTACGACATCGGGTTTTG CTTGGTAGCGCGTTTGTTTT 50.0 45.0 57.9 57.9 249-253 bp 1.0 83.1
trnW-CCA-trnP-UGG_p5 ACCTACGACATCGGGTTTTG TTGGTAGCGCGTTTGTTTTG 50.0 45.0 57.9 58.2 248-252 bp 1.0 82.7