Hotspot

rbcL-rpl23

LSC · 56414-56710 · 297 bp

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Pi 0.0117
MarkerSeek score 47.40
Primer available yes
Label rbcL-rpl23

Feature Metadata

label_name
rbcL-rpl23
feature_type
igs
region
LSC
length_bp
297
variable_sites
27
pi
0.0117
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.96
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-rpl23_p1 TCTCATTATTGCCGCGACAA CTCACGATTCCGCCTGTTAA 45.0 50.0 58.0 58.0 1250-1273 bp 0.98 86.7
rbcL-rpl23_p2 TTGTCTCATTATTGCCGCGA CTCACGATTCCGCCTGTTAA 45.0 50.0 58.0 58.0 1253-1276 bp 0.98 86.6
rbcL-rpl23_p3 GAGCTGTATTTGCGAGGGAA CTCACGATTCCGCCTGTTAA 50.0 50.0 58.0 58.0 1333-1356 bp 0.98 86.3
rbcL-rpl23_p4 TTTTTCACTCAGGACTGGGC CTCACGATTCCGCCTGTTAA 50.0 50.0 58.0 58.0 1004-1027 bp 0.64 70.8
rbcL-rpl23_p5 CGATCAAATTCGAGTTCGCG CTCACGATTCCGCCTGTTAA 50.0 50.0 58.0 58.0 705-723 bp 0.2 56.2