Hotspot

ndhC-trnV-UAC

LSC · 50351-51249 · 899 bp

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Pi 0.00326
MarkerSeek score 55.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
899
variable_sites
34
pi
0.00326
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 983-1025 bp 1.0 86.0
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 984-1026 bp 1.0 84.2
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 982-1024 bp 1.0 83.6
ndhC-trnV-UAC_p4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 985-1027 bp 1.0 82.4
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 983-1025 bp 1.0 81.7