Hotspot

trnG-UCC-trnT-GGU

LSC · 13660-15284 · 1625 bp

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Pi 0.00705
MarkerSeek score 37.90
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1625
variable_sites
63
pi
0.00705
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.00
markerseek_score
37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCACGCCATTACTCTACCAC 38.1 55.0 55.5 58.1 1731-1774 bp 1.0 58.1
trnG-UCC-trnT-GGU_p2 GAACGAATCACACTTTTACCACT CCACGCCATTACTCTACCAC 39.1 55.0 57.3 58.1 1733-1776 bp 1.0 57.1
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACGTCTTACCACGCCATTAC 38.1 50.0 55.5 58.0 1739-1775 bp 0.98 56.1
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC ACGTCTTACCACGCCATTAC 39.1 50.0 57.3 58.0 1742-1778 bp 0.98 55.0
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACGTCTTACCACGCCATTAC 39.1 50.0 57.3 58.0 1741-1777 bp 0.98 55.0