Hotspot

trnG-GCC-trnfM-CAU

LSC · 12283-12751 · 469 bp

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Pi 0.00693
MarkerSeek score 27.50
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
469
variable_sites
17
pi
0.00693
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.00
markerseek_score
27.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GTCGACCCAAGCGGATATAC CGAGGCTCATAACCTTGAGG 55.0 55.0 57.9 57.8 605-639 bp 1.0 84.0
trnG-GCC-trnfM-CAU_p2 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 565-599 bp 1.0 83.9
trnG-GCC-trnfM-CAU_p3 GTCGACCCAAGCGGATATAC CAGTTTGGTAGCTCACGAGG 55.0 55.0 57.9 58.3 620-654 bp 1.0 83.5
trnG-GCC-trnfM-CAU_p4 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.1 58.3 580-614 bp 1.0 83.4
trnG-GCC-trnfM-CAU_p5 GTCGACCCAAGCGGATATAC GAGCGGAGTAGAGCAGTTTG 55.0 55.0 57.9 58.4 633-667 bp 0.98 82.0