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psbE-petL

LSC · 107284-108807 · 1524 bp

Back to Stigmatodon

Pi 0.000677

MarkerSeek score 62.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000677
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1630-1671 bp	1.0	78.1
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1628-1669 bp	1.0	78.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.9	58.5	1643-1684 bp	1.0	77.7
psbE-petL_p4	ACTGACGAATAACCAACCCG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.9	58.5	1641-1682 bp	1.0	77.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.2	58.5	1639-1680 bp	1.0	77.1