Hotspot

rbcL-atpB

LSC · 77781-78556 · 776 bp

Back to Stigmatodon

Pi 0.00644

MarkerSeek score 55.50

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 5
pi: 0.00644
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GGTGACATAAGTCCCTCCCT	GGGGAAAAACGACATCCAGT	55.0	50.0	58.1	58.1	914 bp	1.0	78.7
rbcL-atpB_p2	GGTGACATAAGTCCCTCCCT	TGGGCCAATAATTTGAGCGA	55.0	45.0	58.1	57.9	892 bp	1.0	78.4
rbcL-atpB_p3	TGGTGACATAAGTCCCTCCC	GGGGAAAAACGACATCCAGT	55.0	50.0	58.4	58.1	915 bp	1.0	76.3
rbcL-atpB_p4	TGGTGACATAAGTCCCTCCC	TGGGCCAATAATTTGAGCGA	55.0	45.0	58.4	57.9	893 bp	1.0	76.0
rbcL-atpB_p5	GGTGACATAAGTCCCTCCCT	CTGGGCCAATAATTTGAGCG	55.0	50.0	58.1	57.5	893 bp	1.0	75.5