Hotspot

atpH-atpI

LSC · 55353-56024 · 672 bp

Back to Stigmatodon

Pi 0.00595

MarkerSeek score 62.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 4
pi: 0.00595
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	729-1075 bp	1.0	78.6
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	788-1134 bp	1.0	78.3
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	745-1091 bp	1.0	77.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	730-1076 bp	1.0	77.5
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	759-1105 bp	1.0	77.5