Hotspot

psbI-trnS-GCU

LSC · 49225-49348 · 124 bp

Back to Stigmatodon

Pi 0.0161

MarkerSeek score 60.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 2
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CAATCCTGGACGTGAGGAAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.3	58.0	214 bp	1.0	74.9
psbI-trnS-GCU_p2	CAATCCTGGACGTGAGGAAT	TAGGAGAGATGGCTGAGTGG	50.0	55.0	57.3	57.9	236 bp	1.0	74.5
psbI-trnS-GCU_p3	CAATCCTGGACGTGAGGAAT	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.3	57.6	228 bp	1.0	71.8
psbI-trnS-GCU_p4	CAATCCTGGACGTGAGGAAT	GAGAGATGGCTGAGTGGACT	50.0	55.0	57.3	58.5	233 bp	1.0	71.0
psbI-trnS-GCU_p5	CAATCCTGGACGTGAGGAAT	CTAAAGCGGCGGATTGCTAA	50.0	50.0	57.3	58.7	215 bp	1.0	69.5