Hotspot

matK-trnK-UUU

LSC · 45106-45929 · 824 bp

Back to Stigmatodon

Pi 0.00122

MarkerSeek score 60.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAACGC	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.9	57.9	1032-1039 bp	1.0	78.9
matK-trnK-UUU_p2	CCATGATCATGAGCAAACGC	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.9	57.9	965-972 bp	1.0	78.9
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	TTTGATAGGGTCGTGCAGTG	50.0	50.0	57.8	57.9	982-989 bp	1.0	78.6
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCCGAG	ATCCGGGTTGCTAACTCAAC	50.0	50.0	57.8	57.9	915-922 bp	1.0	78.5
matK-trnK-UUU_p5	CCATGATCATGAGCAAACGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	947-954 bp	1.0	78.4