Hotspot

ndhF

SSC · 113878-116130 · 2253 bp

Back to Stewartia

Pi 0.00616

MarkerSeek score 45.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 78
pi: 0.00616
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGGTTTCGACCAACAAACA	AGGCCAAGTTATTGACCTTAGT	45.0	40.9	58.2	57.1	2699-2833 bp	0.904762	56.0
ndhF_p2	AATCGACCGTTGCGATACAT	AGGCCAAGTTATTGACCTTAGT	45.0	40.9	57.8	57.1	2912-2944 bp	0.857143	53.3
ndhF_p3	GAATCGACCGTTGCGATACA	AGGCCAAGTTATTGACCTTAGT	50.0	40.9	58.4	57.1	2913-2945 bp	0.857143	51.5
ndhF_p4	TGGGTTTCGACCAACAAACA	TGACGGATATTAGAGTCCTTCCT	45.0	43.5	58.2	57.9	2817-2951 bp	0.809524	50.9
ndhF_p5	TGGGTTTCGACCAACAAACA	GTGACGGATATTAGAGTCCTTCC	45.0	47.8	58.2	57.9	2818-2952 bp	0.809524	50.4