Hotspot

lhbA-trnG-UCC

LSC · 38625-38908 · 284 bp

Back to Stewartia

Pi 0.00739

MarkerSeek score 57.20

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 284
variable_sites: 12
pi: 0.00739
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	436-441 bp	1.0	80.8
lhbA-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	434-439 bp	1.0	80.4
lhbA-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	AAATTACTTCACCCTGGGGC	50.0	50.0	57.8	57.8	477-482 bp	1.0	80.3
lhbA-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	AAATTACTTCACCCTGGGGC	55.0	50.0	58.3	57.8	475-480 bp	1.0	80.0
lhbA-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	409-414 bp	1.0	76.6