Hotspot

trnS-GCU-trnG

LSC · 9347-10026 · 680 bp

Back to Stewartia

Pi 0.00464

MarkerSeek score 53.80

Primer available yes

Label trnS-GCU-trnG

Feature Metadata

label_name: trnS-GCU-trnG
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 18
pi: 0.00464
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	791-817 bp	1.0	68.7
trnS-GCU-trnG_p2	CTTTAGTCCACTCAGCCACC	AACCGAAAGACCCCTTAACT	55.0	45.0	57.9	56.4	776-802 bp	1.0	68.3
trnS-GCU-trnG_p3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	840-866 bp	1.0	67.7
trnS-GCU-trnG_p4	TTTAGTCCACTCAGCCACCT	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	775-801 bp	1.0	66.9
trnS-GCU-trnG_p5	ATTAGCAATCCGCCGCTTTA	AAACCGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	792-818 bp	1.0	66.8