Hotspot

trnK-UUU-rps16

LSC · 4336-5138 · 803 bp

Back to Stewartia

Pi 0.00751
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
803
variable_sites
25
pi
0.00751
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 972-982 bp 1.0 74.1
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 973-983 bp 1.0 74.1
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 977-987 bp 1.0 74.1
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 975-985 bp 1.0 74.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 923-933 bp 1.0 73.3