Hotspot

ycf1

SSC · 124023-127184 · 3162 bp

Back to Stevenia

Pi 0.0337

MarkerSeek score 60.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3162
variable_sites: 106
pi: 0.0337
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	AGAGCTTGAATCAGATAAAGAA	45.0	31.8	58.0	52.9	191 bp	1.0	44.2
ycf1_p2	TCCATCGCAATTGAAAACGC	AGAGCTTGAATCAGATAAAGAAA	45.0	30.4	58.0	53.8	191 bp	1.0	44.2
ycf1_p3	TCCATCGCAATTGAAAACGC	AGTAGAGCTTGAATCAGATAAAGA	45.0	33.3	58.0	55.1	194 bp	1.0	44.1
ycf1_p4	TCCATCGCAATTGAAAACGC	AAGTAGAGCTTGAATCAGATAAAGA	45.0	32.0	58.0	55.8	195 bp	1.0	44.1
ycf1_p5	TCCATCGCAATTGAAAACGC	AGTAGAGCTTGAATCAGATAAAGAA	45.0	32.0	58.0	55.8	194 bp	1.0	44.1