Hotspot

rpl16

LSC · 80873-82391 · 1519 bp

Back to Stevenia

Pi 0.0323

MarkerSeek score 64.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1519
variable_sites: 48
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGCCCCAAAAACTCGAGT	GTATGGAGGGAATTGCACGT	45.0	50.0	58.0	58.0	2589-2613 bp	1.0	82.4
rpl16_p2	ATTGCCCCAAAAACTCGAGT	TGCACGTGTTGAATGGATCA	45.0	45.0	58.0	58.1	2237-2261 bp	1.0	82.2
rpl16_p3	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2378-2402 bp	1.0	82.0
rpl16_p4	ATCGGCGATTACTAGCTCCT	TGCACGTGTTGAATGGATCA	50.0	45.0	58.1	58.1	2026-2050 bp	1.0	81.9
rpl16_p5	ATTGTCTCAATTCCCGTGGG	GTATGGAGGGAATTGCACGT	50.0	50.0	57.9	58.0	2609-2633 bp	1.0	81.4