Hotspot

petD-rpoA

LSC · 77394-77593 · 200 bp

Back to Stevenia

Pi 0.065

MarkerSeek score 72.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 13
pi: 0.065
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCTGTGGTTAGGTATTGG	ATTGAACATTTTCACATAGAAGA	55.0	26.1	57.9	52.4	326-332 bp	1.0	43.2
petD-rpoA_p2	GCCCTGTGGTTAGGTATTGG	TTGAACATTTTCACATAGAAGAT	55.0	26.1	57.9	52.4	325-331 bp	1.0	43.2
petD-rpoA_p3	GGCCCTGTGGTTAGGTATTG	ATTGAACATTTTCACATAGAAGA	55.0	26.1	57.9	52.4	327-333 bp	1.0	43.2
petD-rpoA_p4	GGCCCTGTGGTTAGGTATTG	TTGAACATTTTCACATAGAAGAT	55.0	26.1	57.9	52.4	326-332 bp	1.0	43.2
petD-rpoA_p5	CCGTCTTTTTGATTGGCACC	TTGAACATTTTCACATAGAAGAT	50.0	26.1	57.9	52.4	351-357 bp	1.0	42.9