Hotspot

clpP-psbB

LSC · 71598-72087 · 490 bp

Back to Stevenia

Pi 0.0269

MarkerSeek score 73.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 13
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTCCAGGACTTCGAAAAGGT	GAACAGAAAGCAAACGACCG	50.0	50.0	57.2	58.0	600-624 bp	1.0	75.6
clpP-psbB_p2	CTCCAGGACTTCGAAAAGGT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.2	58.2	553-577 bp	1.0	74.1
clpP-psbB_p3	CTCCAGGACTTCGAAAAGGT	CAAACGACCGGGATCATTCA	50.0	50.0	57.2	58.3	590-614 bp	1.0	73.7
clpP-psbB_p4	TCTCCAGGACTTCGAAAAGG	GAACAGAAAGCAAACGACCG	50.0	50.0	56.9	58.0	601-625 bp	1.0	73.3
clpP-psbB_p5	GTCGCACTATATGTCAACCCA	GAACAGAAAGCAAACGACCG	47.6	50.0	57.8	58.0	635-659 bp	1.0	72.8