Hotspot

rbcL-accD

LSC · 56439-57108 · 670 bp

Back to Stevenia

Pi 0.0435

MarkerSeek score 70.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 29
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ATATGCCTGCTTTGACCGAG	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1191-1192 bp	1.0	82.9
rbcL-accD_p2	TCATGGTATGCACTTCCGTG	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1430-1431 bp	1.0	82.6
rbcL-accD_p3	TGCAATGCACGCTGTTATTG	ATTCCGAATGTCCTTGGAGC	45.0	50.0	58.0	58.0	1463-1464 bp	1.0	82.5
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	ATTCCGAATGTCCTTGGAGC	50.0	50.0	58.0	58.0	1630-1631 bp	1.0	82.4
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	1474-1475 bp	1.0	82.2