Hotspot

atpB-rbcL

LSC · 54175-54980 · 806 bp

Back to Stevenia

Pi 0.033

MarkerSeek score 70.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 24
pi: 0.033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	817-894 bp	1.0	77.5
atpB-rbcL_p2	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	816-893 bp	1.0	77.3
atpB-rbcL_p3	ATCGAAACCTCTGGATCCGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	819-896 bp	1.0	76.3
atpB-rbcL_p4	ATCGAAACCTCTGGATCCGA	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.2	58.1	818-895 bp	1.0	76.1
atpB-rbcL_p5	CGAAACCTCTGGATCCGAAG	ACACCAGCTTTGAATCCAACA	55.0	42.9	58.1	58.3	818-895 bp	1.0	75.0