Hotspot

trnT-ACA-trnL-UUA

LSC · 46019-46850 · 832 bp

Back to Stevenia

Pi 0.0278
MarkerSeek score 75.60
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
832
variable_sites
22
pi
0.0278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 854-893 bp 1.0 83.4
trnT-ACA-trnL-UUA_p2 TATGGAAAAGCCGGCTATCG CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 905-944 bp 1.0 82.8
trnT-ACA-trnL-UUA_p3 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 857-896 bp 1.0 82.7
trnT-ACA-trnL-UUA_p4 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 886-925 bp 1.0 82.3
trnT-ACA-trnL-UUA_p5 TATGGAAAAGCCGGCTATCG AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 908-947 bp 1.0 82.1