Hotspot

trnG-GGC-trnfM-AUG

LSC · 36254-36395 · 142 bp

Back to Stevenia

Pi 0.101
MarkerSeek score 75.30
Primer available yes
Label trnG-GGC-trnfM-AUG

Feature Metadata

label_name
trnG-GGC-trnfM-AUG
feature_type
igs
region
LSC
length_bp
142
variable_sites
12
pi
0.101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGC-trnfM-AUG_p1 GTTCGATTCCCGCTATCCG CTTGAGGTCACGGGTTCAAA 57.9 50.0 58.2 58.0 176-197 bp 1.0 79.3
trnG-GGC-trnfM-AUG_p2 GGTTCGATTCCCGCTATCC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 177-198 bp 1.0 79.1
trnG-GGC-trnfM-AUG_p3 GTTCGATTCCCGCTATCCG GGGTAGAGCAGTTTGGTAGC 57.9 55.0 58.2 58.0 212-233 bp 1.0 78.6
trnG-GGC-trnfM-AUG_p4 GGGTTCGATTCCCGCTATC GGGTAGAGCAGTTTGGTAGC 57.9 55.0 57.8 58.0 214-235 bp 1.0 78.4
trnG-GGC-trnfM-AUG_p5 GGTTCGATTCCCGCTATCC GGGTAGAGCAGTTTGGTAGC 57.9 55.0 57.8 58.0 213-234 bp 1.0 78.4