Hotspot

trnH-CAC-psbA

LSC · 77-314 · 238 bp

Back to Stevenia

Pi 0.0641

MarkerSeek score 75.10

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 15
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	311-353 bp	1.0	83.0
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	314-356 bp	1.0	82.9
trnH-CAC-psbA_p3	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	312-354 bp	1.0	82.8
trnH-CAC-psbA_p4	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	315-357 bp	1.0	82.6
trnH-CAC-psbA_p5	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	304-346 bp	1.0	81.5