Hotspot

ndhF

SSC · 112712-114934 · 2223 bp

Back to Sternbergia

Pi 0.0122

MarkerSeek score 55.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 27
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGGTATTGGGAAAAGAAGAGCA	GCGATAAGAATTGGTGAATCGG	45.5	45.5	58.3	57.9	2325-2362 bp	1.0	45.8
ndhF_p2	TGGGTATTGGGAAAAGAAGAGC	GCGATAAGAATTGGTGAATCGG	45.5	45.5	58.3	57.9	2326-2363 bp	1.0	45.8
ndhF_p3	GGGTATTGGGAAAAGAAGAGC	GCGATAAGAATTGGTGAATCGG	47.6	45.5	56.6	57.9	2325-2362 bp	1.0	44.8
ndhF_p4	GGGTATTGGGAAAAGAAGAGCA	CGAAAGCGATAAGAATTGGTGA	45.5	40.9	58.3	57.1	2330-2367 bp	1.0	41.3
ndhF_p5	GGGTATTGGGAAAAGAAGAGCA	TCGAAAGCGATAAGAATTGGTG	45.5	40.9	58.3	57.1	2331-2368 bp	1.0	41.3