Hotspot

ccsA

SSC · 116972-117937 · 966 bp

Back to Sternbergia

Pi 0.00518

MarkerSeek score 47.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 5
pi: 0.00518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.9	58.0	1699-1700 bp	1.0	80.0
ccsA_p2	TAGACACGCTGCTCTTAGGA	TTCTTGGTGGGAACGAGTTG	50.0	50.0	57.9	58.0	1801-1802 bp	1.0	79.8
ccsA_p3	TAGACACGCTGCTCTTAGGA	CTCGATGGCTTCTCTTGCAT	50.0	50.0	57.9	58.0	1697-1698 bp	1.0	79.7
ccsA_p4	TAGACACGCTGCTCTTAGGA	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	1683-1684 bp	1.0	79.6
ccsA_p5	TAGACACGCTGCTCTTAGGA	GAATGAGTGGTTTCGTTGCG	50.0	50.0	57.9	58.0	1671 bp	0.5	58.6