Hotspot

psaC-ndhE

SSC · 120038-120516 · 479 bp

Back to Sternbergia

Pi 0.0373

MarkerSeek score 80.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 479
variable_sites: 17
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	GGAAGCAGCTATTGGGCTAG	50.0	55.0	58.1	58.1	666-672 bp	1.0	82.9
psaC-ndhE_p2	CAATACATCTGTGGGGCAGG	GGAAGCAGCTATTGGGCTAG	55.0	55.0	58.3	58.1	642-648 bp	1.0	81.5
psaC-ndhE_p3	CCAATACATCTGTGGGGCAG	GGAAGCAGCTATTGGGCTAG	55.0	55.0	58.3	58.1	643-649 bp	1.0	81.5
psaC-ndhE_p4	CAGGCTCGTACACATTGAGT	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	626-632 bp	1.0	81.2
psaC-ndhE_p5	AGCTTTACATCCGTCCCAAG	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	672-678 bp	1.0	80.6