Hotspot

ccsA-ndhD

SSC · 117938-118168 · 231 bp

Back to Sternbergia

Pi 0.0736

MarkerSeek score 84.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 17
pi: 0.0736
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGGCACTTGTAGCT	TGACAAAGTGCAGGCTATCC	50.0	50.0	58.6	57.9	393-394 bp	1.0	77.4
ccsA-ndhD_p2	ATTCGGCACTTGTAGCTTCT	TGACAAAGTGCAGGCTATCC	45.0	50.0	57.2	57.9	390-391 bp	1.0	76.4
ccsA-ndhD_p3	ACGAATTCGGCACTTGTAGC	TGACAAAGTGCAGGCTATCC	50.0	50.0	58.9	57.9	394-395 bp	1.0	75.1
ccsA-ndhD_p4	CGAATTCGGCACTTGTAGCT	GACAAAGTGCAGGCTATCCT	50.0	50.0	58.6	57.6	392-393 bp	1.0	75.1
ccsA-ndhD_p5	CGAATTCGGCACTTGTAGCT	CGGATTTCGTTCTCTCGCTA	50.0	50.0	58.6	57.5	418-419 bp	1.0	74.4