Hotspot

rpoC2

LSC · 15505-19620 · 4116 bp

Back to Sterigmostemum

Pi 0.000972

MarkerSeek score 54.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4116
variable_sites: 4
pi: 0.000972
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGTGAACAATGCACCAATCC	TATTACGAGCAGAACGGACG	50.0	50.0	58.0	57.5	259 bp	1.0	76.5
rpoC2_p2	CCGTGAACAATGCACCAATC	TATTACGAGCAGAACGGACG	50.0	50.0	58.0	57.5	260 bp	1.0	76.4
rpoC2_p3	TGGTATGTTGCCGTGAACAA	TATTACGAGCAGAACGGACG	45.0	50.0	58.0	57.5	270 bp	1.0	76.3
rpoC2_p4	TGTTGGTATGTTGCCGTGAA	TATTACGAGCAGAACGGACG	45.0	50.0	58.0	57.5	273 bp	1.0	76.3
rpoC2_p5	TTGGTATGTTGCCGTGAACA	TATTACGAGCAGAACGGACG	45.0	50.0	58.0	57.5	271 bp	1.0	76.3