Hotspot

ccsA

SSC · 113860-114846 · 987 bp

Back to Sterigmostemum

Pi 0.00304

MarkerSeek score 60.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 3
pi: 0.00304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ACCCAACTTTTTCAAAATCGGG	CCTGATTCAAAAGGTTCTTGA	40.9	38.1	57.9	54.0	1170 bp	1.0	40.2
ccsA_p2	CCCAACTTTTTCAAAATCGGGT	CCTGATTCAAAAGGTTCTTGA	40.9	38.1	57.9	54.0	1169 bp	1.0	40.2
ccsA_p3	ACCCAACTTTTTCAAAATCGGG	CCTGATTCAAAAGGTTCTTGAT	40.9	36.4	57.9	54.6	1170 bp	1.0	40.2
ccsA_p4	CCCAACTTTTTCAAAATCGGGT	CCTGATTCAAAAGGTTCTTGAT	40.9	36.4	57.9	54.6	1169 bp	1.0	40.2
ccsA_p5	CCCAACTTTTTCAAAATCGGGT	CCTGATTCAAAAGGTTCTTGATT	40.9	34.8	57.9	55.4	1169 bp	1.0	40.2