Hotspot

atpB

LSC · 52416-53912 · 1497 bp

Back to Sterigmostemum

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	GCGCGTGATTTGGTAATACG	ACCAACGAAATCGAGTGCTT	50.0	45.0	58.0	58.1	1724 bp	1.0	79.0
atpB_p2	ATTCGATTCGGAGTCAGCAC	ACCAACGAAATCGAGTGCTT	50.0	45.0	58.1	58.1	1647 bp	1.0	78.5
atpB_p3	CGGAGTCAGCACACAAAGAT	ACCAACGAAATCGAGTGCTT	50.0	45.0	58.2	58.1	1639 bp	1.0	77.4
atpB_p4	GATTCGGAGTCAGCACACAA	ACCAACGAAATCGAGTGCTT	50.0	45.0	58.2	58.1	1643 bp	1.0	77.4
atpB_p5	ATTCGGAGTCAGCACACAAA	ACCAACGAAATCGAGTGCTT	45.0	45.0	57.7	58.1	1642 bp	1.0	77.0