Hotspot

rpoA-rps11

LSC · 78456-78524 · 69 bp

Back to Sterigmostemum

Pi 0.0145
MarkerSeek score 60.80
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
LSC
length_bp
69
variable_sites
1
pi
0.0145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 ACTTCCACTGTAGTGTCCGA CTGGGAAGAGATGCAGCATT 50.0 50.0 58.0 57.9 238 bp 1.0 79.8
rpoA-rps11_p2 TTCCACTGTAGTGTCCGAGT CTGGGAAGAGATGCAGCATT 50.0 50.0 58.0 57.9 236 bp 1.0 79.8
rpoA-rps11_p3 CACTTCCACTGTAGTGTCCG CTGGGAAGAGATGCAGCATT 55.0 50.0 57.9 57.9 239 bp 1.0 79.4
rpoA-rps11_p4 TTCCACTGTAGTGTCCGAGT AGAGATGCAGCATTACGAGC 50.0 50.0 58.0 58.1 230 bp 1.0 79.2
rpoA-rps11_p5 TTCCACTGTAGTGTCCGAGT GAGATGCAGCATTACGAGCT 50.0 50.0 58.0 58.1 229 bp 1.0 79.2