Hotspot

rpoB-trnC-UGC

LSC · 25883-26923 · 1041 bp

Back to Sterigmostemum

Pi 0.0029

MarkerSeek score 61.10

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 3
pi: 0.0029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1122-1129 bp	1.0	78.9
rpoB-trnC-UGC_p2	AACCCCTGCTCAATAAACCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1188-1195 bp	1.0	78.7
rpoB-trnC-UGC_p3	CCCCTGCTCAATAAACCGAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1186-1193 bp	1.0	77.2
rpoB-trnC-UGC_p4	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1104-1111 bp	1.0	76.7
rpoB-trnC-UGC_p5	AACCCCTGCTCAATAAACCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	1170-1177 bp	1.0	76.5