Hotspot

ccsA

SSC · 121931-122902 · 972 bp

Back to Sterculia

Pi 0.0123

MarkerSeek score 54.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 18
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGTGGCGGCATAGTATCTTC	CGCTGTCCTATGTTAGTATTTGG	50.0	43.5	57.2	57.1	1121-1122 bp	1.0	43.5
ccsA_p2	GTGGCGGCATAGTATCTTCT	CGCTGTCCTATGTTAGTATTTGG	50.0	43.5	57.2	57.1	1120-1121 bp	1.0	43.5
ccsA_p3	GAGTGGCGGCATAGTATCTT	CGCTGTCCTATGTTAGTATTTGG	50.0	43.5	57.2	57.1	1122-1123 bp	1.0	43.4
ccsA_p4	CGAGTGGCGGCATAGTATC	CGCTGTCCTATGTTAGTATTTGG	57.9	43.5	57.9	57.1	1123-1124 bp	1.0	41.3
ccsA_p5	GAGTGGCGGCATAGTATCTTC	CGCTGTCCTATGTTAGTATTTGG	52.4	43.5	58.3	57.1	1122-1123 bp	1.0	41.3