Hotspot

ndhF-rpl32

SSC · 119204-120495 · 1292 bp

Back to Sterculia

Pi 0.027

MarkerSeek score 44.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1292
variable_sites: 24
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	TTTGATGTAGAAGTACGTTTTT	50.0	27.3	58.0	52.0	688-1378 bp	1.0	46.1
ndhF-rpl32_p2	GGGAAGTGGCACAAAAGGTA	CCTTTCTTTTTCCAAACATTTT	50.0	27.3	58.0	52.2	724-1414 bp	1.0	46.0
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	CCTTTCTTTTTCCAAACATTTTT	50.0	26.1	58.0	53.1	724-1414 bp	1.0	46.0
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	CCTTTCTTTTTCCAAACATTTT	45.0	27.3	58.0	52.2	772-1462 bp	1.0	45.9
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	CCTTTCTTTTTCCAAACATTTTT	45.0	26.1	58.0	53.1	772-1462 bp	1.0	45.9