Hotspot

trnN-GUU-ndhF

IRb · 116434-116998 · 565 bp

Back to Sterculia

Pi 0.153

MarkerSeek score 76.50

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 565
variable_sites: 107
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ACGACCAGTCAGTTAACAGC	ATATGTAGGGGGAAGTCGCA	50.0	50.0	57.8	57.9	611-705 bp	1.0	90.0
trnN-GUU-ndhF_p2	ACGACCAGTCAGTTAACAGC	TATGTAGGGGGAAGTCGCAT	50.0	50.0	57.8	57.9	610-704 bp	1.0	90.0
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	ATATGTAGGGGGAAGTCGCA	50.0	50.0	58.2	57.9	624-718 bp	1.0	90.0
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	TATGTAGGGGGAAGTCGCAT	50.0	50.0	58.2	57.9	623-717 bp	1.0	90.0
trnN-GUU-ndhF_p5	ACGACCAGTCAGTTAACAGC	GGGGAAGTCGCATCTCTTTT	50.0	50.0	57.8	57.9	603-697 bp	1.0	89.9