Hotspot

psaI-pafII

LSC · 65476-65895 · 420 bp

Back to Sterculia

Pi 0.0134

MarkerSeek score 53.50

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 8
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	551-563 bp	1.0	82.8
psaI-pafII_p2	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	554-566 bp	1.0	82.7
psaI-pafII_p3	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	561-573 bp	1.0	82.2
psaI-pafII_p4	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	57.9	57.9	564-576 bp	1.0	82.2
psaI-pafII_p5	ACTTTCAGCAGCTTTCCCTC	GGATAAAGGCCCAGCAGAAA	50.0	50.0	58.1	57.9	598-610 bp	1.0	81.9