Hotspot

ndhC-trnV-UAC

LSC · 55625-57013 · 1389 bp

Back to Sterculia

Pi 0.0357

MarkerSeek score 55.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1389
variable_sites: 63
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTACCATAAAGAAGCGGCCA	GGTTGATCTAGGCCAGAAGC	50.0	55.0	58.0	58.0	2080-2223 bp	1.0	86.1
ndhC-trnV-UAC_p2	TGGTACCATAAAGAAGCGGC	GGTTGATCTAGGCCAGAAGC	50.0	55.0	58.0	58.0	2082-2225 bp	1.0	86.1
ndhC-trnV-UAC_p3	CAATGCTCCTTTTCGCCATG	GGTTGATCTAGGCCAGAAGC	50.0	55.0	58.1	58.0	1886-2029 bp	1.0	85.9
ndhC-trnV-UAC_p4	AGGTTCGGGCATTTGTTCAT	GGTTGATCTAGGCCAGAAGC	45.0	55.0	58.1	58.0	2255-2398 bp	1.0	85.6
ndhC-trnV-UAC_p5	GTACCATAAAGAAGCGGCCA	TAGCCTGACAAATGGTTCGG	50.0	50.0	58.0	57.9	2306-2449 bp	1.0	85.5