Hotspot

psbZ-trnG-GCC

LSC · 37986-38995 · 1010 bp

Back to Sterculia

Pi 0.0448

MarkerSeek score 64.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 52
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCCCCTGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	964-1168 bp	1.0	83.7
psbZ-trnG-GCC_p2	CTTCCCCTGATGGTTGGTTG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.5	57.8	960-1164 bp	1.0	83.1
psbZ-trnG-GCC_p3	TTCCCCTGATGGTTGGTTGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.8	57.8	959-1163 bp	1.0	79.9
psbZ-trnG-GCC_p4	TTTGCTTCCCCTGATGGTTG	TACCATTCGACCATATCCGC	50.0	50.0	58.4	57.3	937-1141 bp	1.0	79.3
psbZ-trnG-GCC_p5	CTTCCCCTGATGGTTGGTTG	TACCATTCGACCATATCCGC	55.0	50.0	58.5	57.3	933-1137 bp	1.0	78.7