Hotspot

trnS-GCU-trnG-UCC

LSC · 8140-9038 · 899 bp

Back to Sterculia

Pi 0.0379

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 47
pi: 0.0379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	903-968 bp	1.0	59.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	902-967 bp	1.0	59.7
trnS-GCU-trnG-UCC_p3	CGCACAACGGATTAGCAATC	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	918-983 bp	1.0	59.0
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.3	906-971 bp	1.0	58.7
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	905-970 bp	1.0	58.7