Hotspot

rpl3

SSC · 90449-91072 · 624 bp

Back to Stephanodiscus

Pi 0.0118

MarkerSeek score 53.90

Primer available yes

Label rpl3

Feature Metadata

label_name: rpl3
feature_type: gene
region: SSC
length_bp: 624
variable_sites: 11
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl3_p1	CGTTTAGCAAGTTGGCCAAC	TTTACCTCCTCCACGAACCT	50.0	50.0	58.2	58.0	1365 bp	1.0	79.6
rpl3_p2	GTTGTTCGAAAACCTTCCGC	TTTACCTCCTCCACGAACCT	50.0	50.0	58.2	58.0	1305 bp	1.0	79.5
rpl3_p3	CGTTTAGCAAGTTGGCCAAC	AATGGAGAACGGCTTGATCC	50.0	50.0	58.2	58.0	1424 bp	1.0	79.3
rpl3_p4	GTTGTTCGAAAACCTTCCGC	AATGGAGAACGGCTTGATCC	50.0	50.0	58.2	58.0	1364 bp	1.0	79.3
rpl3_p5	CGTTTAGCAAGTTGGCCAAC	CCCCACCTCTCCATAATGGA	50.0	55.0	58.2	58.2	1438 bp	1.0	78.2